To identify and characterize the mechanisms of biotransformation and drug resistance present in salmonids and in Caligus rogercresseyi, which are involved in the loss of sensitivity of this parasite to the antiparasitic drugs used in Chile for its control.

1.- Determinate the presence (expression and enzymatic activity levels) of the Multidrug Resistance Proteins Pgp, Mrp1, MRP2 and MRP3 in caligus and tissues from Rainbow trout and Atlantic salmon. 2.- Determinate the presence (expression and enzymatic activity levels) of the Cytochrome P450 CYP1A, CYP2, CYP3A, esterases, FMOs and GSTs enzymes in caligus. 3.- Determinate the impact of Emamectin treatment on MDR protein activity and expression levels in caligus and tissues from Rainbow trout and Atlantic salmon. 4.- Determinate the impact of Deltamethrin treatment on expression and activity levels on MDR proteins and the Cytochrome P-450 enzymes in caligus.